Protein levels of catalase and CuZn SOD were significantly increased in
eNOS+/− microvascular tissue. (a) Microvascular tissue from
18-month-old eNOS+/− and wild type animals was Western blotted using
anti-catalase, anti-CuZn SOD, anti-EC SOD, anti-Mn SOD, and anti-Actin (loading
control) antibodies. Representative image is shown. Densitometric analysis was
performed for (b) catalase (n = 9 animals;
**P < 0.01), (c) CuZn SOD (n = 7 animals;
**P < 0.01), (d) EC SOD (n = 5 animals), (e)
Mn SOD (n = 6 animals). All data are presented as mean ±SD with
individual data points shown.