Figure 1.

miR‐124‐3p.1 is upregulated in breast cancer tissues and promotes breast cancer cell growth in vitro. A, Increased level of miR‐124‐3p.1 was positively correlated with pathology status by RT‐qPCR. (r ² = .83, P = .000, n = 76). Pathology status 1‐9 represents Stage 0, Stage IA, Stage IB, Stage IIA, Stage IIB, Stage IIIA, Stage IIIB, Stage IIIC, and Stage IV, respectively. B, The expression level of miR‐124‐3p.1 in BT20 cells that were treated with miR‐124‐3p mimic or miR‐124‐3p inhibitor. C, The effect of miR‐124‐3p.1 on BT20 cell growth was measured by CCK‐8. BT20 cells were treated with miR‐124‐3p mimic or miR‐124‐3p inhibitor. D, E, The effect of miR‐124‐3p.1 on the cell proliferation of BT20 cells was investigated using colony formation assay. The representative results of colony formation of BT20 cells (D) and the quantitative result (E) were shown. F, The effect of miR‐124‐3p.1 on the cell proliferation of BT20 cells was further analyzed by EdU staining. BT20 cells were incubated with EdU for 2 h; then, EdU‐positive cells were detected and counted. (G) The effect of miR‐124‐3p.1 on HCC70 cell growth was measured by CCK‐8. HCC70 cells were treated with miR‐124‐3p mimic or miR‐124‐3p inhibitor. H, I, The effect of miR‐124‐3p.1 on the cell proliferation of HCC70 cells was investigated using colony formation assay. The representative results of colony formation of HCC70 cells (H) and the quantitative result (I) were shown. *P < .05, **P < .01, ***P < .001