Figure 3.

miR‐124‐3p.1 directly inhibits the expression of Axis inhibition protein 1 (Axin1) via targeting its 3′‐untranslated region (3′‐UTR). A, Sequence alignment between miR‐124‐3p.1 and the 3′‐UTR of Axin1 mRNA predicted by TargetScan. B, The effect of miR‐124‐3p.1 on the activity of luciferase reporter containing either wild‐type (WT) or the mutated (Mut) Axin1 3′UTR was examined by luciferase reporter gene assays. 293T cells were transected with WT or the Mut Axin1 3′UTR luciferase reporter plasmids, and the control mimic or miR‐124‐3p.1 mimic. After 24 h, the luciferase activity was detected. (C and D) The effect of miR‐124‐3p.1 on the endogenous expression levels of Axin1 was examined in BT20 cells by RT‐qPCR (C) and Western blotting (D). **P < .01, ***P < .001