Fig. 4.

γ-PGA induced maturation of DCs was TLR4-dependent. BMDCs isolated from wild-type (C57BL/6) and MyD88^−/− mice were stimulated with 100 ng/ml LPS or 1 mg/ml γ-PGA for 12 h, and then the expression of surface molecules, CD40, CD80, and CD86 (a) was analyzed by flow cytometry and concentration of IL-12p70 (b) was measured by ELISA. The results are expressed as mean ± SE of three independent experiments. BMDCs from wild-type (C3H/HeN) or TLR4-defective (C3H/HeJ) mice were stimulated with LPS or γ-PGA, and then expression of surface molecules (c) was analyzed by flow cytometry and concentration of IL-12p70 (d) in culture supernatants was measured by ELSIA. Filled histogram and dotted line indicated γ-PGA treated BMDCs and untreated control (media only), respectively. Asterisks represent not detected. All data are representative of at least three experiments