Fig. 6.
The therapeutic antitumor effect of γ-PGA is mediated by TLR4-dependent and DCs play an important role in antitumor immunity of γ-PGA. C3H/HeN and C3H/HeJ mice were inoculated s.c. with 2.5 × 106 K1735 tumor cells into the left inguinal region. Three days after the inoculation, mice were randomly divided and treated with daily oral treatment of 400 μg γ-PGA (10 or 2,000 kDa) or PBS. Tumor size of C3H/HeN (a) or C3H/HeJ (b) mice was measured twice a week. The data presented in this figure are representative of duplicate experiments. 2.5 × 106 K1735 cells were injected s.c. into the left inguinal region of C3H/HeJ mice. Approximately 7 days after the inoculation and when the size of tumors was about 50 mm2, the mice were randomly divided into six groups consisting of five mice with almost equal mean tumor sizes. iDCs (1 × 106), derived from C3H/HeN (c) or C3H/HeJ (d) mice, were intratumorally injected on days 7, 14, 21, and 28 after tumor inoculation and PBS, 150 μg of 10 kDa γ-PGA, or 150 μg of 2,000 kDa γ-PGA were intratumorally injected on days 8, 11, 15, 18, 22, 25, 29, and 32