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. Author manuscript; available in PMC: 2021 Jan 1.
Published in final edited form as: Methods Mol Biol. 2020;2058:133–154. doi: 10.1007/978-1-4939-9794-7_9

Figure 5: Confirmation of surface expression of the transgene (4-1BBL) in B16-F10 infected cells by flow cytometry.

Figure 5:

B16-F10 cells were infected with NDV-WT or rNDV-4-1BBL at an MOI of 2. Twenty-four hours later, the cells were collected using Cell Stripper, processed for staining of surface 4-1BBL and analyzed by flow cytometry. Blue: NDV-WT, red: rNDV-m4-1BBL.