Table II.

Sublingual immunization with HPV VLP elicits neutralizing Abs in cervicovaginal secretion^a

	Neutralizing Ab Titer in Genital Secretionsb
Immunogen	Geometric mean	Range
Sham	<20	NAc
CT	<20	NAc
HPV16 L1 VLP (s.l.)	46	20–80
HPV16 L1 VLP + CT (s.l.)	61	20–320
HPV16 L1 VLP + alum (i.m.)	32	20–80

^aMice were immunized on days 0 and 7 by the s.l. route with saline (sham), 2μg of CT only, 5μg of HPV16 L1 VLP alone or together with 2 μg of CT. A group of mice was immunized i.m. with 5 μg of HPV16 L1 VLP in alum. Vaginal washes were collected 2 wk after the second immunization and assayed for neutralization activity.

^bDetermined on groups of five mice.

^cNot applicable