Figure 3.

Human IL-33trap specifically targets active IL-33 splice variants (A) Schematic representation of human IL-33 splice variants and encoded protein isoforms. (B) NF-κB activation induced by different IL-33 splice variants. (C) Effect of IL-33trap on NF-κB activation induced by different IL-33 splice variants. (D) Effect of the presence of IL-33 splice variants on the ability of IL-33trap to inhibit IL-33 induced NF-κB activation. NF-κB activity was measured in HEK293T cells as described in Material and Methods. Values represent means ± SE of technical triplicates. Results are representative of at least two independent experiments.