. 2020 Aug;48(8):645–654. doi: 10.1124/dmd.120.090696

TABLE 2.

Kinetic parameters (mean ± S.D.) of UGT1A1, UGT1A9, and UGT2B7 in HLM suspensions and HLM-MGBS

Parameter	Estradiol (UGT1A1)		Parameter	Propofol (UGT1A9)		AZT (UGT2B7)
Parameter	HLM^AS	HLM-MGBS^AS	Parameter	HLM^SI	HLM-MGBS^SI	HLM^MM	HLM-MGBS^MM
S₅₀ (µM)	13 ± 1	6.8 ± 0.6^sig	K_m (µM)	30 ± 4	13 ± 2^sig	1400 ± 0.1	490 ± 0.06^sig
V_max (pmol/min per milligram)	761 ± 34	600 ± 25^sig	V_max (pmol/min per milligram)	2029 ± 49	1635 ± 66^sig	614 ± 21	1366 ± 42^sig
CL_int (µl/min per milligram)	59	88	CL_int (µl/min per milligram)	68	126	0.44	2.8

AS, allosteric sigmoidal modeling used; MM, Michaelis-Menten modeling used; ns, not significantly different from parameter generated using HLM; SI, substrate inhibition modeling used; sig, significantly different from parameter generated using HLM (P < 0.05).