(A) These are representative histologic sections at the distracted area stained with safranin-O/fast green. (B) The mean and SD for areas of the cartilage tissue and newly formed bone at distracted area at Weeks 0, 2, and 4 are shown. At Week 2, the cartilage area was larger in the CO2 group than in the control group. The CO2 group showed greater newly formed bone area (shown are mean and SD) than did the control group at Week 4. Ca = cartilage, Nb = newly formed bone, Cb = cortical bone, Bar = 1 mm.