Table 5. Main IHC markers for the stromal cells.
| MARKERS | NEGATIVE/LOW | INTERMEDIATE | POSITIVE | |
|---|---|---|---|---|
| MACROPHAGES | CD68 (N: 178) | 5 (2.8%) | 124 (69.7%) | 49 (27.5%) |
| CD163 (N:180) | 12 (6.7%) | 99 (55%) | 69 (38.3%) | |
| PDL1 (N:176) | 83 (47.2%) | NA | 93 (52.8%) | |
| CYTOTOXIC | Granzyme (N:178) | 82 (46.1%) | 73 (41%) | 23 (12.9%) |
| Perforin (N:110) | 91 (82.7%) | 3 (2.7%) | 16 (14.6%) | |
| TIA1 (N:177) | 41 (23.2%) | 93 (52.5%) | 43 (24.3%) | |
| T-CELLS | CD3 (N:175) | 64 (36.6%) | 60 (34.3%) | 51 (29.1%) |
| CD4 (N:181) | 84 (46.4%) | NA | 97 (53.6%) | |
| CD8 (N:180) | 66 (36.7%) | NA | 114 (63.3%) | |
| FOXP3 (N:177) | 92(52%) | 9 (5.1%) | 76 (42.9%) | |
| ROR gamma (N:180) | 153 (85%) | 4 (2.2%) | 23 (12.8%) | |
| PD1 (N:176) | 95 (54%) | NA | 81(46%) | |
| B-CELLS | CD20 (N:177) | 128 (72.3%) | NA | 49 (27.7%) |
NA: Not available. Most markers were read as Negative-Low/Intermediate/Positive. A few markers were just dichotomised into Negative/Positive (PDL1 stroma, CD4, CD8, CD20, PD1)