Fig 2. Medium of HEK293-ANKH^wt cells contains large amounts of nucleoside monophosphates.

LC/MS-based global metabolite profiling was applied to 24-hour medium samples of HEK293 parental, HEK293-ANKH^wt and HEK293-ANKH^L244S cells. The relative abundance of masses corresponding to AMP (A), CMP (B), GMP (C) and UMP (D) were determined. Authentic standards were used to confirm the identity of NMPs. Data are expressed as mean +/- SD of an experiment performed in triplicate. Statistical significance was determined by one-way ANOVA with * p < 0.05, ** p < 0.01.