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Acute CED in an orthotopic PDX DIPG rodent model. A: Formation of DIPG was assessed with weekly 3D BLI. B: After the tumor had reached an appropriate size, a CED cannula was inserted and placement was confirmed by CT imaging superimposed on maximal BLI. C: Microscopic evaluation after acute CED administration of a 20-kDa infusate confirmed Vd and distribution across the entire tumor region.
