Key resources table.

Reagent type (species) or resource	Designation	Source or reference	Identifiers	Additional information
Gene Mus musculus	mslo1		GenBank GI: 47143
Gene Mus musculus	Core-MT	Dr. Lawrence Salkoff		PMID:24067659
Biological sample (Xenopus laevis)	oocyte	Xenopus laevis		Xenopus laevis purchased from Nasco, Fort Atkinson, WI
Commercial assay or kit	mMESSAGE T7 Transcription Kit	Thermo Fisher	AM1344
Software, algorithm	Igor Pro 4.0	WaveMetrics	https://www.wavemetrics.com/products/igorpro
Sequence-based reagent	For site-directed mutagenesis	This paper	PCR primers	PCR primers seq for mutations made in this study (each mutation utilized two primers: b and c). K0 b: gCtctGCTgTActtGgaCCCcTtgccgTaGCGGTTTCCTATTAACTC c: cCaagTAcAGCagaGcTgtctccAagggGCACATTGTAGTCTGTG K1 b: cTtGtAcgaCCCGccaTaCTTgttGccgatCcTTAACTCTATGATTTCAG c: ggCGGGtcgTaCaAgAGCGCtGTccGcaagAGcggGCACATTGTAGTCTG K2 b: gagTAGctaCCgCCcTtgTagTTcttgcgTCCTATTAACTCTATGATTTC c: gGGcGGtagCTActcCGCcagggtctcAgGAAAGCACATTGTAGTC K4 b: ctTAgcGGAGtaCgaGccTccgTaGtttcTTCCTATTAACTCTATGATTT c: gCtcGtaCTCCgcTAagaaGGTTAGgaGcAaAggGCACATTGTAGTCTGT K7 b: CTAacGGcGCtgtaGctTccCccGtaGTTTCCTATTAACTCTATG c: gCtacaGCgCCgtTAGgaaGagTaagGGAAGAAAGCACATTGTAG K0 on Core-MT b: gCtctGCTgTActtGgaCCCcTtgccgTaGCGGTTTCCTATTAACTC c: cCaagTAcAGCagaGcTgtctccAagggtGGAGTCAAGGAATCATTA K7 on Core-MT b: gaaGagTaagGGAAGAAAGGGAGTCAAG c: GAAGAAAGGGAGTCAAGGAATCAT K2 on Core-MT b: CCTTGACTCCtTTTCcTgagaccctgG c: gGAAAaGGAGTCAAGGAATCATTATG K0 Y330G b: GCCGccGCGGTTTCCTATTAACTC c: GAAACCGCggCGGCAAGGGGTCCAAG