Table 1. C-linker scrambling mutations and measured V_0.5 in the full-length at both 0 [Ca²⁺] and 100 μM [Ca²⁺] and Core-MT BK channels at 0 [Ca²⁺].

The Core-MT constructs are based on the TMD, C-linker of mSlo1, and an 11-residue tail from K_V 1.4 of the mouse Shaker family (Budelli et al., 2013; Zhang et al., 2017). The location of the nearest Tyr to the S6 C-terminal is underlined. K0 (Y330G) was designed to remove the Tyr sidechain in the K0 background.

Mutation	Sequence	V0.5 (mV)
		Full-Length		Core-MT
		0 [Ca2+]	100 [Ca2+]	0 [Ca2+]
WT	EIIEL IGNRK KYGGS YSAVS GRK	183.4	0.2	235.0
K0	EIIEL IGNRY GKGSK YSRAV SKG	89.6	−66.7	192.6
K0(Y330G)	EIIEL IGNRG GKGSK YSRAV SKG	169.8	47.5	NM
K1	EIIEL RIGNK YGGSY KSAVR KSG	136.2	−4.2	NM
K2	EIIEL IGRKN YKGGS YSARV SGK	195.5	59.0	263.5
K3	EIIEL IGNYG GRSYS KAKVS RKG	NC	NC	NM
K4	EIIEL IGRNY GGSYS AKKVR SKG	94.6	−50.4	NM
K5	EIIER LIGKK RNYKG GSYSA VSG	NC	NC	NM
K6	EIIEL RKKIR KGNYG GSYSA VSG	NC	NC	NM
K7	EIIEL IGNYG GSYSA VRKSK GRK	48.7	−63.7	167.5
	NC: no current, channel could not be expressed; NM: Mutation Not Made