Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Saccharomyces cerevesiae) | Saccharomyces cerevisiae, CVy61 (W303-1a) | doi:10.1002/yea.1406 | ||
| Gene (Saccharomyces cerevesiae) | RIF1 | NA | YBR275C | |
| Gene (Saccharomyces cerevesiae) | DBF4 | NA | YDR052C | |
| Gene (Saccharomyces cerevesiae) | CDC7 | NA | YDL017W | |
| Gene (Saccharomyces cerevesiae) | MCM4 | NA | YPR019W | |
| Gene (Saccharomyces cerevesiae) | MCM5/mcm5-bob1 | gift from John Diffley: doi: 10.1038/nature09373 | YLR274W | |
| Gene (Saccharomyces cerevesiae) | SLD3/sld3-A | gift from John Diffley: doi: 10.1038/nature09373 | YGL113W | |
| Antibody | anti-FLAG (mouse monoclonal) | Sigma-Aldrich | Sigma-Aldrich:F3165; RRID:AB_259529 | (1:1000) |
| Antibody | anti-V5 (mouse monoclonal) | Thermo Fisher Scientific | Thermo:R960-25; RRID:AB_2556564 | (1:2000) |
| Antibody | anti-HA (mouse monoclonal) | Sigma-Aldrich (Roche) | Sigma-Aldrich:11583816001; RRID:AB_514505 | (1:3000) |
| Antibody | anti-MYC (mouse monoclonal) | Santa Cruz Biotechnology | Santa Cruz:sc-40; RRID:AB_627268 | (1:3000) |
| Antibody | anti-PGK1 (mouse monoclonal) | Thermo Fisher Scientific | Thermo:459250; RRID:AB_2532235 | (1:10000) |
| Antibody | anti-Mouse IgGHRP (horse) | Cell Signaling Technology | Cell Signaling:7076; RRID:AB_330924 | (1:10000) |
| Chemical compound, drug | Hydroxyurea | US Biological | US Biological: H9120 | 200 mM |
| Chemical compound, drug | Alpha Factor | Sigma or US Biological | Sigma:T6901; US Biological:Y2016 | 25 ng/ml for bar1∆, 8 µg/ml +6 µg/ml for BAR1 |
| Chemical compound, drug | Sytox Green | Invitrogen | Invitrogen:S7020 | 0.5 µl/ml (2.5 µM final) |
| Software, algorithm | FlowJo 10.6.1 | https://www.flowjo.com | RRID:SCR_008520 | |
| Software, algorithm | Adobe Illustrator CS6 | https://www.adobe.com/products/illustrator.html | RRID:SCR_010279 | |
| Software, algorithm | GE Healthcare Life Sciences ImageQuant v8.1 | https://www.gelifesciences.com/en/us/shop/protein-analysis/molecular-imaging-for-proteins/imaging-software/imagequant-tl-8-1-p-00110 | RRID:SCR_018374 | |
| Software, algorithm | GraphPad Prism 5 | www.graphpad.com | RRID:SCR_002798 | |
| Software, algorithm | RStudio 1.2.1335 | www.rstudio.com/ | RRID:SCR_000432 | heatmap.2 https://www.rdocumentation.org/packages/gplots/versions/3.0.3/topics/heatmap.2 |
| Software, algorithm | SnapGene 5.0.8 | www.snapgene.com | RRID:SCR_015052 | |
| Software, algorithm | CellQuest Pro 5.2.1 | https://www.bdbiosciences.com/documents/15_cellquest_prosoft_analysis.pdf | RRID:SCR_014489 | |
| Software, algorithm | Bedtools Multicov | https://bedtools.readthedocs.io/en/latest/ | RRID:SCR_006646 | doi: 10.1002/0471250953.bi1112s47 |
| Software, algorithm | Samtools | http://www.htslib.org | RRID:SCR_002105 | |
| Software, algorithm | BWA-MEM | https://github.com/lh3/bwa | RRID:SCR_010910 | |
| Software, algorithm | Picard MarkDuplicates | https://broadinstitute.github.io/picard/ | RRID:SCR_006525 | |
| Strains | All strains | Supplementary file 1 | ||
| Recombinant DNA reagent | All plasmids | Supplementary file 1 | ||
| Sequence based reagent | All primers and oligos | Supplementary file 1 | ||
| Cloning reagents | Cells and restriction enzymes | Supplementary file 1 |