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. Author manuscript; available in PMC: 2020 Jul 20.
Published in final edited form as: Cell Rep. 2020 Jun 30;31(13):107828. doi: 10.1016/j.celrep.2020.107828

Figure 6. JTE-013 Inhibits AT2 to AT1 Cell Transition In Vivo.

Figure 6.

(A) Schematic of inducible AT2 cell lineage tracing mice SpC/Tomato.

(B) Four Tmx injections were given to SpC/Tomato mice at 15 to 5 days before PA injury. JTE-013 or DMSO was i.t. injected into the lungs 3 days after PA, and the lungs were harvested on day 10 after the PA.

(C and D) Lung sections were stained for Tomato to mark AT2 and AT2-derived cells. Fewer AT1-like Tomato+ cells (arrows) were detected in JTE-013-treated lungs (D) than control lungs (C). Scale bar, 30 μm. (C′) Enlarged image of a representative lung section showing squamous Tomato+ cells (arrows) co-expressing T1α. Scale bar, 30 μm.

(E and F) Pixel numbers of Tomato+AT1-like cells (E) were measured and normalized to the number of Tomato+ AT2 cells on the same section and plotted (F).

Mean ± SEM. **p < 0.01. See also Figure S6.