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. 2020 Jul 16;12:5845–5855. doi: 10.2147/CMAR.S248698

Figure 4.

Figure 4

MiR-15a is the connection between MCM3AP-AS1 and EIF4E. (A) Predicted binding site of miR-15a on the 3ʹ-UTR of EIF4E mRNA based on the TargetScan database. (B) Western blot analysis was conducted to detect the expression levels of EIF4E, transfected with miR-15a mimic, inhibitor and NC. *P<0.05, ***P<0.0001. (C) Double luciferase reporter assay results of the interaction between miR-15a with the 3ʹ-UTR of EIF4E mRNA. **P<0.01. (D) Predicted miR-15a binding sites in MCM3AP-AS1 sequence based on the DIANA-LncBase analysis. (E) The expression levels of miR-15a was measured by qPCR after transfected with MCM3AP-AS1 siRNA for 48 hours. *P<0.05, **P<0.01. (F) The double luciferase reporter assay experiments investigating the binding affinity of miR-15a to MCM3AP-AS1. *P<0.05, **P<0.01. (G) Cell viability assay of miR-15a mimic or inhibitor on lymphoma cells. *P<0.05. (H) Daudi and Namalwa cells were treated as (G), then apoptosis rates were measured using flow cytometry. *P<0.05, **P<0.01.