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. 2020 Jul 14;12:5759–5771. doi: 10.2147/CMAR.S245283

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© 2020 Qiu et al.

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Effects of ISOV combined with MnSOD silencing and overexpression on migration, invasion and the EMT phenotype. MnSOD and FoxM1 protein levels (A), cell migratory and invasive capacities (B and C), and E-cadherin, N-cadherin, Twist1 and MMP-2 protein amounts (D and E) in HCSLCs transfected with Ad-GFP or Ad-shMnSOD, and incubated with or without ISOV(10 μM) (n=3; *P<0.05 vs Ad-GFP; ^#P<0.05 vs Ad-GFP plus10.0 μM ISOV; one-way ANOVA) are shown. MHCC97H cells were transfected with Ad-GFP or Ad-MnSOD, and incubated with ISOV (10 μM). MnSOD and FoxM1 protein levels (F), cell migratory and invasive capacities (G and H), and E-cadherin, N-cadherin, Twist1 and MMP-2 protein amounts (I and J) in MHCC97H cells transfected with Ad-GFP or Ad-MnSOD, incubated with or without ISOV(10 μM) (n=3; *P<0.05 vs Ad-GFP; ^#P<0.05 vs Ad-GFP plus 10.0 μM ISOV; ^&P<0.05 vs Ad-MnSOD alone; one-way ANOVA) are shown.