Skip to main content
. 2020 Jul 16;5(4):1113–1126. doi: 10.1016/j.bioactmat.2020.07.002

Fig. 7.

Fig. 7

miR-1246 in DPSC-Exo facilitates macrophages to convert from a pro-inflammatory phenotype to an anti-inflammatory phenotype in vitro. (A) Relative proportion of miRNAs in the total miRNA reads. (B) miR-1246 levels in DPSC-Exo and DPSCs were analysed by RT-qPCR (n = 6 per group). Error bars represent SEM. *p < 0.05. (C) Representative density plots of phenotypic characterization of BMDMs treated with PBS, DPSC-Exo or DPSC-Exo + miR-1246 inhibitor. Gates captured single, live CD11b+F4/80+ cells. Statistical analysis of the flow cytometry data describing the MFI of CD206+ cells (n = 3). Error bars represent SEM. *p < 0.05. (D) Representative density plots of phenotypic characterization of BMDMs treated with PBS, DPSC-Exo or DPSC-Exo + miR-1246 inhibitor. Statistical analysis of the flow cytometry data describing the MFI of CD86+ cells (n = 3). Error bars represent SEM. *p < 0.05. (E) Representative density plots of phenotypic characterization of BMDMs treated with PBS or miR-1246 mimics. Gates captured single, live CD11b+F4/80+ cells. Statistical analysis of the flow cytometry data describing the MFI of CD206+ cells (n = 3). Error bars represent SEM. *p < 0.05. (F) Representative density plots of phenotypic characterization of BMDMs treated with PBS or miR-1246 mimics. Statistical analysis of the flow cytometry data describing the MFI of CD86+ cells (n = 3). Error bars represent SEM. *p < 0.05.