Extended Data Fig. 5. The ribose subunit of inosine can replace glucose and feed into the central carbon metabolism in T effector cells.
a, The same experiment as in Fig. 2 was performed for active human T cells and the quantification of indicated metabolites were analyzed by IC-UHR-FTMS. Data are presented as mean ± SEM (n=3). Numbers in the X-axis represent those of 13C atoms in given metabolites. b, Quantification of the metabolites in the dual-tracer experiment with [13C5]-Inosine and [6,6-D2]-Glucose. All symbols and abbreviations are as described in Fig. 2. The X-axis represents unlabeled (0), 13C-labeled carbon (Cx), and Deuterium (Dx) in given metabolites. Values represent mean ± SEM (n=3). c, OCR/ECAR of active human T cells cultured in glucose versus inosine conditional media. Values represent mean ± SD (n=5). Data are representative of two independent experiments. Sample size (n) represents biologically independent samples (a–c).