Extended Data Fig. 10. Inosine supplement fails to enhance immunotherapy in MC-38, TrampC2 and M3-9-M tumor models.
(a–c) Murine xenograft model was established in C57BL/6 mice by subcutaneous inoculation of indicated tumor cells. The indicated experimental mice were treated with IgG control (200 μg, i.p. twice/week), inosine (300 mg/kg, oral gavage every day), anti-PDL1 or anti-PD1 antibody (200 μg, i.p. twice/week), and anti-PDL1 or anti-PD1 antibody (200 μg, i.p. twice/week) + inosine (300 mg/kg, oral gavage daily). Tumor size and mouse survival were monitored. Data are presented as mean ± SEM (n=7, 9, 9, 10 for IgG Control, Inosine, Anti-PDL1, Anti-PDL1+Inosine for MC-38; n=7, 10, 9, 10 for IgG Control, Inosine, Anti-PDL1, Anti-PDL1+Inosine for TrampC2; n= 8, 9, 9, 9 for IgG Control, Inosine, Anti-PD1, Anti-PD1+Inosine for M3-9-M). Sample size (n) represents biologically independent tumors. Tumor progression curves (left) were analyzed with Two-Way ANOVA and mouse survival curves (right) were analyzed with one-sided Mantel-Cox test. NS, P=0.4544, 0.9999, 0.3601 for antibody versus antibody+inosine for tumor progression curves of MC-38, TrampC2, and M3-9-M, respectively. NS, P=0.1255, 0.8822; *, P=0.0390 for antibody versus antibody+inosine for survival curves of MC-38, TrampC2, and M3-9-M, respectively. Data are representative of two independent experiments (a–c).