Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;10:11991. doi: 10.1038/s41598-020-68770-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Gating Strategy and Microglia Identification at 24 h after Injury. Microglial cells were identified using the following gating strategy: cells were identified as the main population (with the orange population representing the counting beads), and then debris and cell aggregates were excluded using correlation of FSC area vs height. Dead cells were excluded using Ghost™ dye. For the visual comparison, microglia were identified as CD45⁺CD11b/c⁺P2y12⁺ cells. Panel A is representing sham ipsilateral while panel B represents CCI ipsilateral samples. Both are presented to imply on the dramatic changes that are observed only from this initial step. The enriched fraction of cells includes neutrophils, monocytes and macrophages that share similar markers to those of resident microglia (CD11bc but not only). Using P2y12 we were able to distinguish microglia (CD45⁺CD11b/c⁺P2y12⁺) from other cell types and characterize each subpopulation of the cells. (C) At 24 h after injury, we conclude, that the main population of cells underwent dramatic changes in size (FSC), granularity (SSC) and in its absolute cells number. We attribute these changes mostly to microglial cells as there are also increased in % from the total population observed. Values presented are mean values ± s.e.m (n = 3).