Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;10:11991. doi: 10.1038/s41598-020-68770-0

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Assessment of Changes in Cell Density and Marker Expression following Injury Using tSNE. The machine-learning algorithm T-distributed Stochastic Neighbor Embedding (tSNE), which uses a nonlinear dimensionality reduction, was applied for visualization of the data. The islands created are determined by the algorithm contain events that share similar properties. The x- and y-axis and unites are determined by the algorithm (A) Presentation of total cell density changes comparing sham and injured with both contra-and ipsi-lateral hemispheres at 24 h after injury. The CCI ipsilateral shows a clear change with the formation of two distinct new islands. (B) Heat-maps of the mean values of the different markers used in the study are presented to visualize the changes the microglial population is undergoing at 24 h after injury. For comparison, the ipsilateral of sham and CCI are presented.