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. 2020 Jul 20;10:11958. doi: 10.1038/s41598-020-67465-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Coronin 1C-null melanoma grows faster than endogenous-expressing tumors. (a) Schematic diagram of two GEM models, PBT and PBT-1C. Upon 4-hydroxytamoxifen application, CreER expressed under the melanocyte-specific Tyrosinase promotor excises the DNA between LoxP sites, denoted with red triangles, resulting in Pten deletion, BRAF constitutive activation and TdTomato expression. In the PBT-1C model, Coro1C is also deleted. (b) Transillumination and red fluorescence for TdTomato imaging of a PBT-1C primary tumor 8 weeks after induction with 4-hydroxytamoxifen. (c) Transillumination of one PBT primary tumor and one PBT-1C primary tumor over a 12-week period following induction. (d) Quantification of the mean thickness + /− 95% CI of the ear and primary tumor (N of 15 for PBT and N of 16 for PBT-1C. (e, f) Representative multi-photon imaging of second harmonic signal of the bundled collagen layer and TdTomato expression in recombined melanocytes in the primary tumor of a PBT mouse ear (e) and a PBT-1C mouse ear (f) over an 8-week period post induction with 4-HT. Scale bars = 50 µm.