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. 2020 Jul 20;10:11996. doi: 10.1038/s41598-020-68947-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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PI-PLC treatment completely abrogated Ly6H suppressible effect on choline-evoked currents in TARO cells. (A) Representative confocal microscopic images of DAPI (blue) for nuclear staining, Cherry (red) for confirming transfection efficiencies, and Ly6H (white) staining in TARO cells treated with or without PI-PLC. Scale bar, 10 μm. (B) Representative whole-cell recordings from indicated cells stimulated with 30 μM choline together with 1 μM PNU120596. (C) Quantification of choline and PNU120596-evoked peak currents with or without PI-PLC treatment. Student’s t test P values : Mock vs non-treatment = *P < 0.05, non-treatment versus PI-PLC treated = **P < 0.01.