Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2020 Jul 20;10:11997. doi: 10.1038/s41598-020-68829-y

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Mps1 inhibition induces mitotic defaults in neuroblastoma cells. (A,B) SK-N-Be2c neuroblastoma cells were transfected with siUNR or siMps1a for 72 h. Cells were fixed for immunofluorescence and cells nuclei were labeled with DAPI for size estimation. Representative picture of cells nuclei are reported in (A) while quantitative data of nucleus size are reported in (B). n represents the number of counted nuclei. (C,D) SK-N-Be2c neuroblastoma cells were treated with DMSO or Reversine, or transfected with siUNR or siMps1a for 72 h. Cells were then fixed for immunofluorescence and labeled with DAPI for DNA detection and anti-α-Tubulin – FITC antibody for the microtubules visualization. For the quantification of mitotic default, mitotic figures were followed. Representative pictures of mitotic default are shown in panel (C) while panel (D) reports quantitative data for mitotic defaults accumulation. Data are reported in SEM n = 3. ***(p < 0.001) indicates significant difference from control (ANOVA).