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. 2020 Jul 20;10:11950. doi: 10.1038/s41598-020-68701-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of normovolemic hemodilution on ALAT (A), ASAT (B), LDH (C), CK (D), CK isoenzymes (E) and K+ (F) in plasma in addition to K+ in urine (G). During hemodilution (grey background), animals were diluted using 5% HSA (control) or 12 vol% capsules (treatment) to a hematocrit of 5%. The plots (A-D, F, G) show the mean ± SEM of n = 8 animals per group. Asterisk indicates significance with p < 0.05 compared to the controls. CK-isoenzyme determination (E) in respectively three plasma samples per group of minute 162 (examined animals). Control animals showed slight bands of CK-BB and CK-MM, whereas the treatment group showed increased bands of CK-BB and CK-MM. Comparison of CK-isoenzyme pattern of the treatment group with the organ-specific pattern of organ homogenates of cerebrum, cerebellum, heart, muscle, spleen, liver, lung, intestinal and kidney of healthy untreated animals matched best with the spleen-specific pattern of CK-isoenzymes treatment bands with organ homogenates.