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. 2020 Jul 20;10:11950. doi: 10.1038/s41598-020-68701-z

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Macrophage staining of the spleen. Immunohistochemical staining with CD68 clone ED1 antibody of spleen tissue of animals diluted using 5% HSA (control) or 12 vol% capsules (treatment). Hematoxylin (blue) co-staining was used for orientation. The brown colour represents the macrophages. Control animals showed unaffected macrophages in contrast to foamy vacuolized macrophages in the treatment group. Staining was performed with n = 8 spleens in each group. Treatment spleens demonstrated lost tissue structure within the red pulpa with foamy, swollen macrophages. The white pulpa was not affected. The control spleens were intact.