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. 2020 Jul 20;10:11950. doi: 10.1038/s41598-020-68701-z

Figure 8.

Figure 8

Quantification of renal EPO-mRNA. Animals were diluted using 5% HSA (control) or 12 vol% capsules (treatment) to a hematocrit of 5%. Kidneys were used for EPO-mRNA in situ hybridization. (A) Number of EPO-positive cells per field of view (FOV) in the in the renal cortex or the renal medulla of the kidney after hemodilution. (B) Representative pictures of the EPO-mRNA in situ hybridization used for quantitative analysis. The brown staining demonstrates the EPO-mRNA. In situ hybridization was performed with n = 8 kidneys in each group. For determination of EPO-positive cells 10 randomly chosen FOV per renal cortex or 5 randomly chosen FOV per medulla were counted.