Table 2.
Effect of Gly and Roundup addition during IVM on fertilization rate, monospermy rate and on the ability of oocytes to sustain male pronucleus formation after in vitro fertilization. Data represent the mean ± SD of six replicates repeated in different experiments.
| 0 | 5 | 10 | 100 | 200 | 360 | |
|---|---|---|---|---|---|---|
| Gly (µg/mL) | ||||||
| Penetration rate | 75.6 ± 4.0 | 73.3 ± 5.2 | 73.5 ± 8.1 | 78.2 ± 5.8 | 78.1 ± 5.2 | 72.7 ± 5.0 |
| Monospermy rate | 65.3 ± 12.2 | 62.8 ± 9.7 | 51.3 ± 12.6 | 60.2 ± 15.6 | 63 .2 ± 13.1 | 53 .5 ± 6.4 |
| Male pronuclear formation | 99.4 ± 1.5 | 99.4 ± 1.6 | 98.5 ± 2.4 | 99.4 ± 1.5 | 99.3 ± 1.7 | 98.5 ± 2.5 |
| Oocytes (n°) | 284 | 284 | 243 | 221 | 244 | 262 |
| Roundup (µg/mL Gly eq) | ||||||
| Penetration rate | 76.0 ± 5.2 | 75.4 ± 4.8 | 74.5 ± 10.1 | 75.6 ± 8.7 | 68.8 ± 10.0 | 69.0 ± 8.9 |
| Monospermy rate | 54.4 ± 11.8 | 56.5 ± 9.9 | 63.7 ± 14.2 | 55.4 ± 11.1 | 66.9 ± 11.0 | 56.9 ± 12.5 |
| Male pronuclear formation | 100.0 ± 0.0 | 98.7 ± 2.0 | 98.8 ± 2.9 | 97.2 ± 3.3 | 98.7 ± 2.1 | 99.5 ± 1.3 |
| Oocytes (n°) | 267 | 227 | 233 | 254 | 228 | 242 |