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. 2020 Jul 20;11(7):551. doi: 10.1038/s41419-020-02746-w

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Western blot analysis of RPS6KB1, p-RPS6KB1, EIF4EBP1, and p-EIF4EBP1. a A549 cells were treated with compound 5e at indicated concentrations for 6 h along with pretreatment of 3BDO or not. The pretreatment time of 3BDO was 3 h and the concentration were 0.1, 1, and 30 μM respectively. b A549 cells were treated with compound 5e for 6 h in 10 μM solely or along with SiFKBP12 in 60 nM for 24 h. β-actin was used as a loading control. Results were presented as mean ± SE; n = 3; *p < 0.05; **p < 0.01.