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. 2020 Jul 20;15:150. doi: 10.1186/s11671-020-03377-y

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a U87MG cell uptake of the TDN and Apt-TDN (TDN-Gint4.T). The TDN entered U87MG cells directly without transfection agents, and the uptake of Apt-TDN (linked to the aptamer Gint4.T) was significantly increased and competitively inhibited by free Apt (Gint4.T), indicating that the aptamer Gint4.T plays a significant role in cellular targeting. The scale bar represents 50 μm. b Flow cytometry curves show the intracellular uptake of the TDN, Apt-TDN, and Apt-TDN+Apt after incubation for 3 h