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. 2020 Jul 20;10:129. doi: 10.1186/s13568-020-01063-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — ELISA analysis. a Shows a calibration curve constructed by twofold dilution series of the purified anti-G17-Gly. The absorbance values and the standard scFv concentrations showed a satisfactory linear correlation considering R² > 0.9. b Represents the overall concentration of the anti-G17-Gly scFv extracts under different experimental conditions. The mean absorbance values of the test samples were interpolated to the linear regression equation obtained from the calibration curve. The Mean OD stands for the average absorbance values of duplicate samples read at 450 nm and 630 nm to subtract background absorbance