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. 2020 Jul 20;10:129. doi: 10.1186/s13568-020-01063-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — Response surface plots associated with the extraction yield of periplasmic anti-G17-Gly scFv. The statistically significant interaction terms effective in the anti-G17-Gly scFv extraction (mg/L) are showed by the response surface plots: a The interaction between incubation time and Tris–HCl concentration (AC); b The interaction between EDTA and pH (BD); c The interaction between EDTA and Tris–HCl concentration (BC); d The interaction between Tris–HCl concentration and pH (CD)