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. 2020 Jul 20;10:129. doi: 10.1186/s13568-020-01063-x

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Characterization of the optimal conditions. a, b display respectively SDS-PAGE and immunoblotting of the anti-G17-Gly scFv expressed at the mentioned optimal conditions. TES extraction shows much purer periplasmic fraction than the TES/lysozyme method. Control is the purified anti-G17-Gly scFv