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. 2020 Jul 20;10:11989. doi: 10.1038/s41598-020-68381-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Figure represents ratio chart obtained through coffalyser.NET showing profile of DMD subject with long stretch deletion between exon 20 and 44. (A, B) Ratio chart and electropherogram depicting exonic deletions from exon 41 to 44 and exon 21 to 30 covered by P034 probe-mix. (C, D) Ratio chart and electropherogram depicting deletions from exon 31 to 40 and exon 20 covered by P035 probe-mix. P034 and P035 covers probes of all 79 exons of the DMD gene. Ratio between 0.70 and 1.30 was considered in the normal range while ratio of 0.00 was considered as deletion (depicted in red dots in ratio chart and red arrows in the electropherogram).