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. 2020 Jul 20;10:11954. doi: 10.1038/s41598-020-68769-7

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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CCL2 overexpression induces histological alterations in the liver and muscle. (A) Representative microphotographs of the liver and muscle sections stained with hematoxylin and eosin (H&E), Sirius red and F4/80 marker. (B) Western blot analyses of clusters of differentiation (CD) 11b, tumor necrosis factor (TNFα), and CD163. Arrowheads indicate hepatic steatosis, and arrows show positive staining for Sirius red (collagen fibers) and F4/80. The results are shown as the mean ± SEM (n = 8). *P < 0.05, with respect to the WT control littermates.