Figure 6.

NKG2D signaling in NOD CD8⁺ T cells increases CD8⁺ T_cm. A: Representative flow cytometry data gated on in vitro activated NOD CD3⁺CD8⁺ cells showing CD44 and CD62L expression. The numbers shown are the percentage of cells present in each quadrant of the dot plot. B and C: The percentage (mean ± SD) of NOD CD8⁺CD3⁺ cells that were CD44⁺CD62L⁺ (T_cm) (B) or CD44⁺CD62L⁻ (T_{eff + em}) (C) after activation in vitro in the presence of RAE1ε+ or RAE1ε− splenocytes. D: The ratio (mean ± SD) of NOD CD8⁺ T_{eff + em}:T_cm generated after activation in vitro in the presence of RAE1ε+ or RAE1ε− splenocytes. These data are representative of three independent experiments. ****P < 0.0001 by two-sided t test.