Table 5.

Concentration (μg/mL) of antimicrobials residues in milk following intramammary administration

Number of antimicrobial intramammary injection	Methods	1st and 2nd injection, 12 h apart		Time (h) after the last antimicrobials administration
		12 h	24 h	12 h	24 h	36 h	48 h	60 h	72 h	84 h	96 h	108 h	120 h	132 h	144 h
Cephalosporin 3rd Generation Ceftiofur IMM 132 mg/quarter	HPLC‐UVa	22.46	1.64	21.56	1.95	0.16	0.03	< 0.015	< 0.015	< 0.015	< 0.015	< 0.015	< 0.015	< 0.015	< 0.015
	HPLC‐DCAb	40.92	83.8	45.05	12.44	4.0	1.88	0.55	0.3	0.13	0.09	0.07	0.04	0.03	0.02

HPLC: high‐performance liquid chromatography; UV: ultraviolet; DCA: desfuroylceftiofur acetamide.

Table adapted from EMA MRLs assessment reports.

^aMethod for the detection of desfuroylceftiofur.

^bMethod for the detection of acetamide derivative of desfuroylceftiofur.