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. 2020 Jul 14;11:1594. doi: 10.3389/fmicb.2020.01594

TABLE 4.

Identification of taxon K isolates using MALDI-TOF MS.

Cell smears identified using MBT IVD library
 Cell extracts identified using MBT IVD library
 Cell extracts identified using MBT IVD + in-house library
Isolate Consensus best match Log score Identification Consensus best match Log score Identification Consensus best match* Log score Identification
 Burkholderia contaminans
B. contaminans LMG 23253 B. cepacia MB_7544_05 2.37 B. cepacia B. cepacia MB_7544_05 2.45 B. cepacia B. contaminans LMG 23361T 2.57 Bcc
B. contaminans LMG 23361T B. cepacia MB_7544_05 2.27 Bcc B. cepacia MB_7544_05 2.41 Bcc B. contaminans LMG 23361T 2.59 Bcc
B. contaminans R-18175 B. cepacia DSM 7288T 2.21 Bcc B. cepacia MB_7544_05 2.36 Bcc B. contaminans LMG 30304 2.72 B. contaminans
B. contaminans R-18430 B. cepacia DSM 7288T 2.28 Bcc B. cepacia DSM 7288T 2.42 Bcc B. contaminans LMG 30304 2.61 B. contaminans
B. contaminans R-18442 B. cepacia DSM 7288T 2.29 Bcc No consensus NA Bcc B. contaminans LMG 30304 2.71 B. contaminans
B. contaminans R-19218 B. cepacia DSM 7288T 2.31 Bcc No consensus NA Bcc B. contaminans R-19218 2.74 B. contaminans
B. contaminans R-37747 B. cepacia MB_7544_05 2.34 Bcc B. cepacia MB_7544_05 2.37 B. cepacia B. contaminans LMG 23361T 2.47 Bcc
Other Bcc C AU33423 B. cepacia DSM 7288T 2.23 Bcc B. cepacia DSM 7288T 2.27 Bcc B. contaminans LMG 31807 2.49 Bcc
Other Bcc C HI2714 B. cepacia DSM 7288T 2.09 Bcc B. cepacia DSM 50181 2.22 Bcc B. contaminans LMG 31808 2.30 Bcc
Other Bcc C LMG 31807 B. cepacia DSM 7288T 2.27 Bcc No consensus NA Bcc B. contaminans LMG 31807 2.74 B. contaminans
Other Bcc G AU15512 B. cepacia MB_7544_05 2.15 Bcc B. cepacia MB_7544_05 2.43 B. cepacia B. contaminans LMG 23361T 2.52 Bcc
Other Bcc G AU27893 B. cepacia DSM 7288T 2.24 Bcc B. cepacia MB_7544_05 2.37 Bcc B. contaminans LMG 31807 2.55 Bcc
Other Bcc G LMG 31808 No consensus NA Bcc B. cepacia DSM 7288T 2.37 Bcc B. contaminans LMG 31808 2.71 B. contaminans
Other Bcc G HI4860 B. cepacia DSM 7288T 2.24 Bcc B. cepacia DSM 7288T 2.36 Bcc B. contaminans LMG 31807 2.55 Bcc
Other Bcc H AU6039 B. cepacia DSM 7288T 2.24 Bcc B. cepacia MB_7544_05 2.31 Bcc B. contaminans LMG 31806 2.57 Bcc
Other Bcc H AU31652 B. lata DSM 23089T 2.24 Bcc B. cepacia MB_7544_05 2.36 Bcc B. contaminans LMG 16227 2.58 Bcc
Other Bcc H HI2500 B. cepacia MB_7544_05 2.24 Bcc B. cepacia MB_7544_05 2.40 Bcc B. contaminans LMG 31808 2.53 Bcc
Other Bcc H LMG 31806 B. cepacia MB_7544_05 2.28 Bcc B. cepacia MB_7544_05 2.46 B. cepacia B. contaminans LMG 31806 2.67 B. contaminans
Other Bcc H R-71171 B. cepacia MB_7544_05 2.32 Bcc B. cepacia MB_7544_05 2.38 Bcc B. contaminans LMG 31806 2.49 Bcc
 Burkholderia lata
B. lata LMG 6860 B. pyrrocinia LMG 14191T 2.26 Bcc B. lata DSM 23089T 2.38 Bcc B. lata LMG 6992 2.49 Bcc
B. lata LMG 6863 B. lata DSM 23089T 2.28 Bcc B. lata DSM 23089T 2.34 Bcc B. lata LMG 6992 2.51 Bcc
B. lata LMG 6992 B. lata DSM 23089T 2.29 Bcc B. pyrrocinia LMG 14191T 2.31 Bcc B. lata LMG 6992 2.50 Bcc
B. lata LMG 6993 B. lata DSM 23089T 2.45 B. lata B. lata DSM 23089T 2.60 B. lata B. lata LMG 6993 2.62 Bcc
B. lata LMG 22485T B. lata DSM 23089T 2.52 B. lata B. lata DSM 23089T 2.61 B. lata B. lata LMG 22485T 2.63 B. lata
B. lata R-18110 B. lata DSM 23089T 2.31 Bcc B. lata DSM 23089T 2.41 Bcc B. aenigmatica sp. nov. R-18628 2.47 Bcc
B. lata R-50215 No consensus NA Bcc B. cepacia DSM 7288T 2.21 Bcc B. lata LMG 14095 2.34 Bcc
B. lata LMG 14095 B. cepacia DSM 9241 2.15 Bcc B. pyrrocinia LMG 14191T 2.17 Bcc B. lata LMG 14095 2.55 B. lata
B. lata R-15816 No consensus NA Bcc B. metallica DSM 23519T 2.20 Bcc B. lata LMG 14095 2.31 Bcc
B. lata R-15945 B. lata DSM 23089T 2.15 Bcc B. metallica DSM 23519T 2.25 Bcc B. lata LMG 14095 2.31 Bcc
B. lata R-18109 B. lata DSM 23089T 2.21 Bcc B. stabilis DSM 16586T 2.22 Bcc B. aenigmatica sp. nov. LMG 31805 2.28 Bcc
B. lata R-18112 B. lata DSM 23089T 2.25 Bcc B. lata DSM 23089T 2.35 Bcc B. aenigmatica sp. nov. LMG 31805 2.40 Bcc
B. lata LMG 23254 B. lata DSM 23089T 2.24 Bcc B. lata DSM 23089T 2.37 Bcc B. lata LMG 23254 2.64 Bcc
B. lata R-39750 B. lata DSM 23089T 2.39 Bcc B. metallica DSM 23519T 2.27 Bcc B. lata LMG 22485T 2.40 Bcc
 Burkholderia aenigmatica sp. nov.
B. lata LMG 31805 B. lata DSM 23089T 2.28 Bcc B. lata DSM 23089T 2.32 Bcc B. aenigmatica sp. nov. LMG 31805 2.62 Bcc
B. lata R-9940 B. cepacia DSM 7288T 2.19 Bcc No consensus NA Bcc B. aenigmatica sp. nov. R-18628 2.65 Bcc
B. lataR-17378 B. cepacia DSM 7288T 2.22 Bcc B. cepacia DSM 9241 2.25 Bcc B. aenigmatica sp. nov. R-18628 2.50 Bcc
B. lata R-18628 B. cepacia DSM 50181 2.20 Bcc B. cepacia DSM 9241 2.33 Bcc B. aenigmatica sp. nov. R-18628 2.73 B. aenigmatica sp. nov.
Other Bcc M AU17325 B. lata DSM 50180 2.24 Bcc B. lata DSM 50180 2.41 Bcc B. aenigmatica sp. nov. LMG 13014T 2.49 Bcc
Other Bcc M LMG 13014 B. cepacia DSM 7288T 2.21 Bcc B. lata DSM 50180 2.45 Bcc B. aenigmatica sp. nov. LMG 13014T 2.68 B. aenigmatica sp. nov.

Consensus best match was reported if the same best matching spectrum was observed for at least half of the replicate spectra (six for cell smears, four for cell extracts). The log score represents the average of log scores corresponding to the best matching spectrum. The identification of the best matching spectrum was accepted in case of a log score ≥2.0 and when a 0.200 log difference was observed between the best matching spectrum and the highest-scoring spectrum of a different species. MBT, MALDI BioTyper; IVD, in vitro diagnostics; ID, identification; Bcc, Burkholderia cepacia complex; NA, not available; ∗, species names in this column correspond to the taxonomic proposals in the present study. For strains indicated in bold type, MSPs were added to the in-house library to improve identification of taxon K bacteria.