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. 2020 Jul 19;13(10):100825. doi: 10.1016/j.tranon.2020.100825

Fig. 3.

Fig. 3

TANs suppress NK cell immunity by PD-L1/PD-1 interaction. (a) Schematic diagram showing the experimental protocol. Neutrophils from normal or tumor-bearing mice were transfused into naïve or tumor-bearing mice, and NK cells were collected for the activation experiments. (b) The effects of neutrophils on NK cell responsiveness. After experimental treatment as described above in (a), the percent of IFN-γ + NK cells were detected by FCM following in vitro stimulation with NK-activating receptor (NKp46 or NKG2D) antibodies or IgG control. (c) The effect of PD-L1 neutralizing antibody on NK cell responsiveness. Neutrophils and NK cells from naïve or tumor-bearing mice were co-cultured with or without neutralizing antibody against PD-L1 for 12 h, and the percent of IFN-γ + NK cells from the spleen of naïve or tumor-bearing mice was detected by FCM following in vitro stimulation with NK-activating receptor (NKp46 and NKG2D) antibodies or IgG control. (d) The effect of PD-1 neutralizing antibody on NK cell responsiveness. Neutrophils and NK cells from naïve or tumor-bearing mice were co-cultured with or without neutralizing antibody against PD-1 for 12 h, and the percent of IFN-γ + NK cells from the spleen of naïve or tumor-bearing mice was detected by FCM following in vitro stimulation with NK-activating receptor (NKp46 and NKG2D) antibodies or IgG control. Results are expressed as mean ± SD from four independent experiments (n = 6 in each group). *p < 0.05, **p < 0.01.