Figure 3.

Focal CAR T Cell Therapy

(A) Schematic of experimental setup. 5- to 6-week-old SCID mice were injected subcutaneously (s.c.) with 1.5 × 10⁶ PSMA⁺ C4-2^luc+ tumor cells. When tumors reached ~70 mm³, mice were injected intratumorally (i.t.) with a single dose of 5 × 10⁶ CAR28 (n = 5) or CAR41 (n = 5) T cells (day 1 of treatment). As controls, mice were left untreated (control, n = 6) or injected with untransduced T cells (UT, n = 7). (B) In vivo imaging. Shown are representative in vivo bioluminescence images (BLIs) of mice in different treatment groups. (C) Quantification of tumor size. Tumor volumes were determined on days 1, 3, 8, 15, and 22 of treatment. (D) Summary. Shown is antitumor response in different treatment groups. CR, complete response; PR, partial response. ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, by unpaired t test.