Figure 3.

Effects of the Deletion of cdvB1 and cdvB2 in Cell Division and Cell Growth in Sulfolobus acidocaldarius

(A) Growth of MW001 (background strain), ΔcdvB1, and ΔcdvB2 on BNS medium plates, showing that the growth of ΔcdvB2 is compromised.

(B) Western blots of MW001, ΔcdvB1, and ΔcdvB2 using CdvB1 and CdvB2 antibodies. The DNA binding protein Alba was used as a loading control.

(C) Time-lapse microscopy showing MW001, ΔcdvB1, and ΔcdvB2 cells. Purple dashed lines encircle cells undergoing successful divisions, whereas cells undergoing failed divisions are encircled by red dashed lines. The blue arrowheads point to small cells that drift across the imaging field.

(D) Time-lapse imaging of ΔcdvB1 cells that following membrane furrowing fail division, resulting in the fusion of daughter cells.

(E) Quantification of the frequency of division failures in immobilized wild-type, MW001, ΔcdvB1, and ΔcdvB2 cells. Five independent movies were used to evaluate the frequency of division failure from 130 to 180 divisions per strain, in total.

Scale bars: 1 μm. Error bars show mean and SD. All live imaging shown in this figure was performed using immobilized cells. See also Figure S2 and Videos S3 and S4.