REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Rabbit Anti-CdvB	[17]	N/A
Chicken Anti-CdvB1	[17]	N/A
Guinea pig Anti-CdvB2	[17]	N/A
Mouse Anti-Alba	[17]	N/A
Goat anti-rabbit IRDye 680CW	LI-COR	Cat# 926-68021; RRID:AB_10706309
Donkey anti-chicken IRDye 800CW	LI-COR	Cat# 926-32218; RRID:AB_1850023
Donkey anti-guinea pig 800CW	LI-COR	Cat# 926-32411; RRID:AB_1850024
Goat anti- mouse IRDye 800CW	LI-COR	Cat# 926-32210; RRID:AB_621842
Goat anti-rabbit Alexa Fluor 546	Invitrogen	Cat# A11035; RRID:AB_143051
Goat anti-guinea pig Alexa Fluor 488	Invitrogen	Cat# A11073; RRID:AB_2534117
Goat anti-chicken Alexa Fluor 488	Invitrogen	Cat# A11039; RRID:AB_142924
Chemicals, Peptides, and Recombinant Proteins
SYBR safe	Thermo Fisher Scientific	Cat# S33102
Nile red	Sigma-Aldrich	Cat #72485
Concanavalin A Alexa Fluor 647 Conjugated	Thermo Fisher Scientific	Cat# C21421
DNase I	Thermo Fisher Scientific	Cat# EN0521
EDTA-free protease inhibitor cocktail	Roche	Cat# 11836170001
Hoechst	Invitrogen	Cat# H3570
Experimental Models: Organisms/Strains
S. solfataricus P2 (Wild type)	DSMZ	DSM1616
S. acidocaldarius DSM639 (Wild type)	DSMZ	DSM639
MW001 Uracil auxotrophic background strain ΔpyrE (Δbp 91–412), background: S. acidocaldarius DSM639	[6]	N/A
ΔcdvB1 (Saci_0451) (background MW001)	This work	N/A
ΔcdvB2 (Saci_1416) (background MW001)	This work	N/A
ΔcdvB2+pSVA (ΔcdvB2 transformed with pSVA empty plasmid)	This work	N/A
ΔcdvB2+pSVA-CdvB2 (ΔcdvB2 transformed with pSVA containing the wild-type gene of CdvB2, under an inducible promoter controlled by Arabinose)	This work	N/A
Recombinant DNA
Plasmid pSVA431 (Backbone of deletion plasmid containing a pyrEF and LacS cassette from S. solfataricus)	[6]	N/A
Plasmid pSVA1895 Used for deletion of cdvB1 (Saci_0451)	This work	N/A
Plasmid pSVA1896 Used for deletion of cdvB2 (Saci_1416)	This work	N/A
Plasmid pSVAaraFX-stop Backbone of expression plasmid harboring an arabinose inducible promoter containing a pyrEF cassette from S. solfataricus	[41]	N/A
Plasmid pSVAaraCdvB2 Used for expression of CdvB2 (Saci_1416)	This work	N/A
Software and Algorithms
Adobe Ilustrator	Adobe Inc.	https://www.adobe.com/products/illustrator
Prism v.8	GraphPad	https://www.graphpad.com/scientific-software/prism/
ImageJ (FiJi)	NIH	https://imagej.nih.gov/ij/
ImageJ plugin StackReg	[42]	http://bigwww.epfl.ch/thevenaz/stackreg
FlowJo v10.1	FlowJo	https://www.flowjo.com/solutions/flowjo