REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Chicken anti-GFP	Abcam	CAT#ab13970; RRID: AB_300798
Rabbit anti-tRFP	Evrogen	CAT#AB233; RRID: AB_2571743
Rabbit anti-Cx43	Sigma-Aldrich	CAT#C6219-1MG; RRID: AB_476857
Anti-chicken Alexa488	Invitrogen	CAT# A-11039; RRID: AB_142924
Rabbit anti-β-Tubulin	Abcam	CAT#ab209866
Rabbit IgG control	Merck	CAT#12-370; RRID: AB_145841
Goat anti-rabbit HRP antibody	Abcam	CAT#ab97080; RRID: AB_10679808
Anti-rabbit-Cy3	Jackson ImmunoResearch	CAT#711-165-152; RRID: AB_2307443
Bacterial and Virus Strains
PAO1 Pseudomonas aeruginosa	Martin Welch	N/A
Chemicals, Peptides, and Recombinant Proteins
Cxcl8a	ProteinTech	N/A
non-hydrolysable ATP-γS	Sigma-Aldrich	CAT#A1388-1MG
SKF 96365	Cayman Chemical	CAT#10009312
P. aeruginosa isolation agar	Scientific laboratory supplies LTD	CAT#257002
Nalidixic acid	Scientific laboratory supplies LTD	CAT#SR102E
DMEM	Invitrogen	CAT# 16219961
FBS	GIBCO ThermoFisher Scientific	CAT# 16140071
Penicillin/Streptomycin	Sigma-Aldrich	CAT#TMS-AB2-C
DAPI	Sigma-Aldrich	CAT#D8417-1MG
Lipofectamine 2000	invitrogen	CAT# 11668019
LTB4	Sigma-Aldrich	CAT#L0517-10UG
NF279	BIO-TECHNE LTD	CAT#1199
U-75302	Cayman Chemical	CAT#70705
MS-222	Sigma-Aldrich	CAT# E10521-50G
2-APB	Tocris Bioscience	CAT#1224
Methylene blue	Sigma-Aldrich	CAT# M9140-25G
1-phenyl-2-thiourea	Sigma-Aldrich	CAT#P7629-25G
EGTA	Sigma-Aldrich	CAT#E3889-25G
Carbenoxolone	Sigma-Aldrich	CAT#C4790-5G
Methanol-free formaldehyde	Thermofisher	CAT#28906
Sudan Black	Sigma-Aldrich	CAT#3080-1KT
PBS	Oxoid	CAT#BR0014G
Tween-20	Sigma-Aldrich	CAT#P-1379
Syto-62	Invitrogen	CAT#S11344
Low melting point agarose	Invitrogen	CAT#16520-100
A23187	Sigma-Aldrich	CAT# C7522-10MG
Critical Commercial Assays
KOD Hot start kit	Novagen, TOYOBO	CAT#71086
SuperScript III Reverse Transcriptase	Invitrogen	CAT#18080051
RNAeasy minikit	QIAgen	CAT#74104
Pierce ECL Plus Western Blotting Substrate	Invitrogen	CAT#32132
Sp6 mMessage mMachine kit	Ambion	CAT#AM1340
Experimental Models: Cell Lines
HEK293T cells	Felix Randow’s laboratory	N/A
Experimental Models: Organisms/Strains
Zebrafish Tg(lyz:GCamp6F)	This paper	N/A
Zebrafish Tg(lyz:cx43 dn-T2A-mCherry)	This paper	N/A
Zebrafish Tg(lyz:tRFP-5LO)	This paper	N/A
Zebrafish Tg(mpx:GFP)i114	[60]	N/A
Zebrafish Tg(lyz:lta4h-eGFP)	This paper	N/A
Zebrafish AB strain	PDN fish facility	N/A
Oligonucleotides
Primer cx43 Forward: 5¢-GCTCTCCA CTCTTTACTTCTTTCCAG-3¢	This paper	N/A
Primer cx43 Reverse: 5¢-GTATTGCACTTGAAAGCTGACTGC-3¢	This paper	N/A
Primer cx43.4 Forward: 5¢-GAGTCGTCATCGCGAGACATTGA-3¢	This paper	N/A
Primer cx43.4 Reverse: 5¢-GTCTATGAGTCTCAATCAAGCATGGATCC-3¢	This paper	N/A
Primer lta4h Forward: 5¢-TCTGAGAAGGAATATGTGGATGAA-3¢	[18]	N/A
Primer lta4h Reverse: 5¢-CAGCAAGAGATCTGTCTCCA-3¢	[18]	N/A
cx43 translation morpholino: 5¢-GTTCTAGCTGGAAAGAAGTAAAGAG-3¢	Gene Tools	ZFIN: ZDB-MRPHLNO-080818-1
cx43.4 Splice Morpholino: 5¢-ACTTCTCCATCTCCGTTATATTTTG-3¢	Gene Tools	N/A
Control Morpholino 5¢-CCTCTTACCTCAGTTACAATTTATA-3¢	Gene Tools	https://www.gene-tools.com/content/negative-control-morpholino-oligos
lta4h splice morpholino: 5¢-CAGTCTGATCAAGAGAAAGACTCGA-3¢	Gene Tools	ZFIN: DB-MRPHLNO-171122-3
Recombinant DNA
GCamp6F	[22]	N/A
lta4h-EGFP	Gene synthesized (Genewiz)	lta4h cDNA: ENSDART00000028171.7
tRFP-5lo	cDNA library from whole adult zebrafish	5lo/alox5a cDNA: ENSDART00000079884.6
cx43dn_T2a_mCherry	(Gene synthesized (Genewiz)	N/A
Cxcl8-mCherry	[30]	N/A
pCS2-TP	[61]	N/A
Software and Algorithms
MATLAB R2018b	https://www.mathworks.com/	N/A
Imaris v8.2	https://imaris.oxinst.com/	N/A
Fiji	https://fiji.sc/	N/A
Other
Bolt 10% Bis-Tris Plus Gel	Invitrogen	CAT#NW00100BOX
1mm glass beads	BioSpec	CAT#11079110
iBlot2 transfer stack	Life Technologies	CAT#IB23001