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. 2020 Jul 17;205(3):708–719. doi: 10.4049/jimmunol.2000089

FIGURE 2.

FIGURE 2.

Differential dependence on T-bet for IFN-γ production in CD4 and CD8 T cells post–-STm infection. Frequency and total numbers of IFN-γ+CD62L−CD4+ T cells in (A) spleen and (B) liver. (C) MFIs of IFN-γ in CD62L−CD4+ T cells from spleen and liver on day 21 postinfection. (D) Quantification of IFN-γ in supernatants from cells obtained from spleens and livers on day 21 postinfection stimulated with anti-CD3 and anti-CD28. (E) Quantification of IFN-γ in supernatants from spleen and liver cells obtained from mice infected for 21 d: nonstimulated (NS), stimulated with anti-CD3 and anti-CD28, or stimulated with 107 heat-killed STm (HK). Each point represents a value for the tissue of a single mouse, and bars show the median value. Data combined from at least two independent experiments. Mann–Whitney U test was applied in (A) and (B). Nonparametric two-way ANOVA with Tukey post hoc test was applied in (C) and (D). *p < 0.05, **p < 0.01, ***p < 0.001. ns, nonsignificant.