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Generation of eGFP-DMNB-Sec39. (a) Fluorescence microscopy and quantification of HEK293T cells transiently transfected with a pAcBac2 plasmid encoding LeuRS BH5 T252A /Leu tRNA/eGFP-39-TAG, in the presence and absence of DMNB-Sec. (b) SDS-PAGE of eGFP-WT and LeuRS BH5 T252A /Leu tRNA/eGFP-39-TAG expressed in the presence and absence of DMNB-Sec (c) ESI-MS analysis of eGFP-DMNB-Sec39 (expected mass: 29865.33) and irradiated eGFP-Sec39 (expected mass: 29669.24)
