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. 2020 Jul 21;217(11):e20201181. doi: 10.1084/jem.20201181

Figure S2.

Figure S2.

Variables determining HIV-1 pseudotype infection signal. (A) Images (GFP) of confluent monolayers of the indicated cell lines after infection with equivalent amounts of CCNanoLuc/GFP pseudotyped with SARS-CoV-2 SΔ19 or no S protein, as indicated. Scale bar, 0.4 mm. (B) Relationship between NanoLuc luciferase activity (RLU) and ACE2 cell surface expression levels (quantified by flow cytometry; Fig. S1 A) following infection the cell lines depicted in Fig. S1 A with HIV-1NLΔEnv-NanoLuc pseudotyped virus. Mean and range of two technical replicates are plotted. (C) Infectivity of CCNanoLuc/GFP pseudotyped virus generated by cotransfection with the indicated amounts of SARS-CoV-2 SΔ19 expression plasmid. Mean and range of two technical replicates are plotted. (D) Quantification of infectivity of HIV-1NL4-3ΔEnv-NanoLuc pseudotype infection by immunostaining of 293T/ACE2(B) target cells with antibodies against the HIV-1 capsid (CA) protein. Nuclei were visualized by Hoechst staining. Scale bar, 70 µm.