Figure 2. Performance of a whole-cell E. coli cyanuric acid sensor across time, optical densities, and CYA concentrations.

(a) Fold-induction of the whole-cell cyanuric acid (CYA) sensor measured as ratio of reporter fluorescence in the presence or absence of 150 μM CYA. The starting cell optical densities (OD₆₀₀) ranged from 0.25 to 16. Timepoints were taken every hour for 12 hours post-induction with CYA, and fold induction reported at all time points. The OD₆₀₀ normalized sfGFP fluorescence time course for starting OD₆₀₀ 0.5 is plotted with and without 150 μM CYA (bottom). The OD₆₀₀ normalized sfGFP fluorescence for starting OD₆₀₀ at 0.5 to 16 at the 3-hour timepoint is plotted with and without 150 μM CYA (right). (b) Dose-response curve of the sensor measured 3 hours post-induction for starting OD₆₀₀ 0.5. The OD₆₀₀ normalized GFP fluorescence was taken from 0 to 150 μM CYA. All measurements were taken on 3 biological replicates. Averages are shown with error bars representing 1 standard deviation.